Manchester United will be without Anderson, Rio Ferdinand, Ryan Giggs, Jonny Evans and Michael Owen for their Champions League tie against Marseille.
Only Anderson played in Saturday's FA Cup victory over Crawley Town and the Brazilian was taken off at half-time.
Skipper Ferdinand has not played since injuring his calf before facing Wolves on 5 February, while fellow centre-back Evans has an ankle problem.
Giggs and Owen were in the squad which played Manchester City on 12 February.
But 31-year-old striker Owen is thought to have a groin injury.
Continuing problems in the heart of United's defence mean that Chris Smalling is likely to start alongside Nemanja Vidic for Wednesday's last-16 tie at the Stade Velodrome.
United manager Sir Alex Ferguson has also included Brazilian twins Rafael and Fabio, despite the pair both coming off with injuries during the 1-0 win over Crawley at Old Trafford.
Youngsters Joshua King and Ryan Tunnicliffe have also been selected in the squad, along with Gabriel Obertan and Bebe.
Marseille qualified for the knockout stages second in Group F behind Chelsea, but are set to be without striker Andre-Pierre Gignac, who was injured last weekend.
Sir Alex Ferguson will address the media on Tuesday to explain the latest injury news for the five that did not make the trip to the south of France.
Manchester United squad: Van der Sar, Kuszczak, Lindegaard; Vidic, O'Shea, Brown, Rafael, Fabio, Evra, Smalling; Scholes, Gibson, Nani, Carrick, Fletcher, Bebe, Obertan, Tunnicliffe; Rooney, Berbatov, Hernandez, King.
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